N.B. IT IS EXPECTED THAT RESEARCHERS ACKNOWLEDGE THE OLSON LAB WEBSITE AND/OR RELEVANT PUBLICATIONS WHEN USING THE DATA OR INFORMATION PROVIDED BELOW
Hymenolepis microstoma genome data
Data from the Hymenolepis microstoma genome sequencing effort at the Wellcome Trust Sanger Institute, based on specimens from our laboratory model, are made publicly available as the data are generated.
WEBSITE
Data from the Hymenolepis microstoma genome sequencing effort at the Wellcome Trust Sanger Institute, based on specimens from our laboratory model, are made publicly available as the data are generated.
WEBSITE
Sequence Alignments
NEXUS-formatted files with sites excluded from published analyses defined as character exclusion sets ('EXSET') in an 'Assumptions' block.
Riddiford N and PD Olson. 2011. Wnt gene loss in flatworms. Development Genes and Evolution 221:187-197 PDF
ALIGNMENT (ALL SITES)
ALIGNMENT (ANALYSED POSITIONS)
Olson PD, JN Caira, K Jensen, H Palm, RM Overstreet and I Beveridge. 2010. Evolution of the trypanorhynch tapeworms: parasite phylogeny supports independent lineages of sharks and rays. International Journal for Parasitology 40:223-242
ALIGNMENT | Supplementary Data S1 | PDF
Olson PD. 2008. Invited Review: Hox genes and the parasitic flatworms: new opportunities, challenges and lessons from the free-living. Parasitology International 57:8-17
ALIGNMENT* | Publication
(*Note: regions outside of the homeodomain used for analysis are not in alignment)
Olson PD, LG Poddubnaya, DTJ Littlewood and T Scholz. 2008. On the position of Archigetes and its bearing on the early evolution of the tapeworms. Journal of Parasitology 94:898-904
ALIGNMENT | Publication
Olson PD, TH Cribb, VV Tkach, RA Bray and DTJ Littlewood. 2003. Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33:733-755
ALIGNMENT | Publication
NEXUS-formatted files with sites excluded from published analyses defined as character exclusion sets ('EXSET') in an 'Assumptions' block.
Riddiford N and PD Olson. 2011. Wnt gene loss in flatworms. Development Genes and Evolution 221:187-197 PDF
ALIGNMENT (ALL SITES)
ALIGNMENT (ANALYSED POSITIONS)
Olson PD, JN Caira, K Jensen, H Palm, RM Overstreet and I Beveridge. 2010. Evolution of the trypanorhynch tapeworms: parasite phylogeny supports independent lineages of sharks and rays. International Journal for Parasitology 40:223-242
ALIGNMENT | Supplementary Data S1 | PDF
Olson PD. 2008. Invited Review: Hox genes and the parasitic flatworms: new opportunities, challenges and lessons from the free-living. Parasitology International 57:8-17
ALIGNMENT* | Publication
(*Note: regions outside of the homeodomain used for analysis are not in alignment)
Olson PD, LG Poddubnaya, DTJ Littlewood and T Scholz. 2008. On the position of Archigetes and its bearing on the early evolution of the tapeworms. Journal of Parasitology 94:898-904
ALIGNMENT | Publication
Olson PD, TH Cribb, VV Tkach, RA Bray and DTJ Littlewood. 2003. Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33:733-755
ALIGNMENT | Publication
Primers
Olson Lab Primers (Geneious Folder archive)
UPDATED 08.11.2009
Olson Lab Primer Spreadsheet (Excel Workbook)
UPDATED 31.10.2009
Includes PCR/RT-PCR/QPCR primer definitions for universal nuclear ribosomal, mitochondrial, Hox and other homeodomain genes in (mostly) parasitic flatworms, especially Hymenolepis microstoma.
Olson Lab Primers (Geneious Folder archive)
UPDATED 08.11.2009
Olson Lab Primer Spreadsheet (Excel Workbook)
UPDATED 31.10.2009
Includes PCR/RT-PCR/QPCR primer definitions for universal nuclear ribosomal, mitochondrial, Hox and other homeodomain genes in (mostly) parasitic flatworms, especially Hymenolepis microstoma.
Protocols
Whole-Mount In-Situ Hybridization (WMISH)
UPDATED 23.11.2010
Recipes for WMISH
UPDATED 23.11.2010
Protocol for WMISH in flatworm parasites using digoxigenin-labelled riboprobes with an alkaline-phosphotase substrate. The protocol is based on those used in the lab of Prof Peter Holland at Oxford and is applicable to a wide range of organisms. Thanks to Nick Riddiford for revising and updating the protocol.
Whole-Mount In-Situ Hybridization (WMISH)
UPDATED 23.11.2010
Recipes for WMISH
UPDATED 23.11.2010
Protocol for WMISH in flatworm parasites using digoxigenin-labelled riboprobes with an alkaline-phosphotase substrate. The protocol is based on those used in the lab of Prof Peter Holland at Oxford and is applicable to a wide range of organisms. Thanks to Nick Riddiford for revising and updating the protocol.