DAY 1: Saturday | 31st January, UQ Aquatic Centre, The University of Queensland
17:00 Welcome Reception
A conference registration desk will be staffed from 17:00 to 19:00
DAY 2: Sunday | 1st February, UQ Centre, The University of Queensland
08:00 Registration & Breakfast (Conference Centre Lobby)
09:15 Introductory Remarks [Conference Chair: Dr Brad Marsh, Institute for Molecular Bioscience, The University of Queensland, Australia]
Official Opening
09:45 Session 1: Visual Proteomics - Integrating biochemical and molecular approaches with high-content molecular characterization by ET
Chair: Dr Stephan Nickell, Max-Planck Institute of Biochemistry, Martinsried, Germany
From Visual Proteomics to Systems Biology
Invited Speakers: Dr Friedrich Förster, University of California at San Francisco, USA (Co-Chair)
Retrieving Molecular
Architectures of Protein Complexes using EM Densities and Proteomics Data
A/Prof. Dorit Hanein, Burnham Institute for Medical Research, La Jolla, USA
Seeing the Structural Organization of the Actin Cytoskeleton at the Lamella
Dr Martin Beck, Institute of Molecular Systems Biology, Department of Biology, ETH, Zurich
Molecular Anatomy of the Human Pathogen Leptospira interrogans
Dr Juergen Plitzko, Max-Planck Institute of Biochemistry, Martinsried, Germany
Merging the Power of Visualization with the Power of Identification – Strategies and Developments in Cryo-Electron Microscopy
Selected Speakers: Rubén Fernández-Busnadiego, Max-Planck Institute of Biochemistry, Martinsried, Germany
Molecular Architecture of the Presynaptic Terminal Studied by Cryoelectron Tomography
11:00 Morning Tea
11:30 Session 1: Cont’d
12:30 Lunch (Kiosk & Verandah)
13:45 Session 2: Pre- and post-processing methods for segmentation, denoising and feature detection of ET datasets
Chair: A/Prof. Niels Volkmann, Burnham Institute for Medical Research, La Jolla, USA
Extracting Residue-Level Information from Electron Tomograms
Invited Speakers: Dr Achilleas Frangakis, EMBL, Heidelberg, Germany (Co-Chair)
Image Analysis of Vitreous Sections
Dr Tom Goddard, University of California at San Francisco, USA
Interactive Visualization of Spikes, Pores and Cages
Dr Sjors Scheres, Centro Nacional de Biotecnología, Universidad Autónoma, Madrid, Spain
Sub-Tomogram Classification Through Likelihood Optimization
Selected Speakers: Fernando Amat, Stanford University, Stanford, USA
Structural Analysis of Caulobacter Crescentus CB15 S-layer by Cryo-Electron Tomography of Whole Cells
Andrew Noske, The University of Queensland, Brisbane, Australia
3D Reconstruction and Analysis of Mammalian Cells In Toto by New Tomographic and Computational Approaches
Peter van der Heide, The University of Queensland, Brisbane, Australia
Towards Whole Cell Imaging at High Resolution - Large Area Electron Tomography of Mammalian (Insulin-Secreting) Cells
15:00 Afternoon Tea
15:30 Session 2: Cont’d
17:00 Poster Session A
A wine and cheese reception will accompany poster viewing from 17:00 to 18:30
DAY 3: Monday | 2nd February, UQ Centre, The University of Queensland
08:00 Breakfast (Lobby)
09:00 Session 3: The best of both worlds: Converging and hybrid ET approaches between materials and life sciences
Chair: Prof. Paul Midgley, University of Cambridge, UK
Recent Developments in Electron Tomography of Materials
Invited Speakers: Dr Matthew Weyland, Monash Centre for Electron Microscopy, Melbourne, Australia (Co-Chair)
Tomography of Precipitates in Aluminium Alloys by High Angle Annular Dark Field (HAADF) Scanning Transmission Electron Microscopy (STEM)
Dr Kenji Kaneko, Kyushu University, Japan
Characterization of Various Crystalline Materials by Three-Dimensional Electron Tomography
Prof. Hiroshi Jinnai, Kyoto Institute of Technology, Japan
Electron Tomography on Soft Materials: A Self-Assembly in a Block Copolymer
Selected Speakers: Jens Leschner, University of Ulm, Germany
Direct Determination of Binarization Parameters for Quantification of Nanoparticles – Comparison of ±90° and ±75° Acquisition Schemes
Zineb Saghi, University of Sheffield, UK
Advances in Electron Tomography for Materials Science
11:00 Morning Tea
11:30 Session 3: Cont’d
12:30 Lunch (Kiosk & Verandah)
13:45 Session 4: Data sharing, integration and computational simulation
Chair: Prof. Maryann Martone, University of California at San Diego, USA
The Cellular Knowledge Base: Ontology-based Annotation and Query of Electron Tomography Data
Invited Speakers: Prof. Jose-Maria Carazo, University of Madrid, Madrid, Spain (Co-Chair)
Tomograpic Data in the Age of Data Integration: How to Share Data and How to Make Sense of Them
Dr Nigam Shah, National Center for Biomedical Ontology, USA
BioPortal – Ontologies and Data Resources with the Click of a Mouse
Dr Christoph Best, EMDB
The Next-Generation Electron Microscopy Data Bank: Rich Content, Quality Assessment and Large Dataset Management
Selected Speakers: Graham Johnson, The Scripps Research Institute, La Jolla, USA
Automated Visualization of Subcellular Environments: The Role of Tomography in the Proteomics Era
Automated Visualization of Subcellular Environments: The Role of Tomography in the Proteomics Era
15:00 Afternoon Tea
15:30 Session 4: Cont'd
17:00 Poster Session B
A wine and cheese reception will accompany poster viewing from 17:00 to 18:30
DAY 4: Tuesday | 3rd February, UQ Centre, The University of Queensland
08:00 Breakfast (Lobby)
09:00 Session 5: Linking the modes: New correlative approaches between light microscopy and electron tomography
Chair: Prof. David Mastronarde, University of Colorado at Boulder, USA
Extending the Size of Tomographic Reconstructions by Stitching together Laterally Adjacent Volumes
Invited Speakers: Dr Thomas Müller-Reichert, Max Planck Institute for Molecular Cell Biology & Genetics, Dresden, Germany (Co-Chair)
Correlative Light Microscopy and Electron Tomography of the Early C. elegans Embryo
Dr Andrew Leis, Max-Planck Institute of Biochemistry, Martinsried, Germany
Visualising the Hydrated Cell Interior Using Photons, Electrons and X-Rays
Dr Rachid Sougrat, National Institutes of Health, Bethesda, USA
Correlative PALM/EM and iPALM/3D EM
Cindi Schwartz, University of Colorado, Boulder, USA
Advances in a Cryo-Light
Microscope Stage for Correlations with Cryo-EM
Selected Speakers: Prof. Leann Tilley, La Trobe University, Melbourne, Australia
Electron and X-Ray Tomography of Malaria Parasite-Infected Erythrocytes
Dr Claude Antony, EMBL, Heidelberg, Germany
Microtubular Network 3D Reconstruction in WT and Mutant Cells in Fission Yeast by Electron Tomography
11:00 Morning Tea
11:30 Session 5: Cont’d
12:30 Lunch (Kiosk & Verandah)
13:45 Session 6: 3D reconstruction of macromolecules and membranes
Chair: A/Prof. Ben Hankamer, Institute for Molecular Bioscience, The University of Queensland, Australia
Towards Multi-Scale Analysis of Photosynthetic Light Capture Processes
Invited Speakers: A/Prof. Steve Ludtke, Baylor College, Houston, USA
High Resolution Single Particle Reconstruction and Synergies with Cryo-ET
Dr Henning Stahlberg, University of California at Davis, USA
Where Electron Crystallography of Membrane Proteins meets Electron Tomography and Single Particle EM
Dr Holger Stark, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
Structure Determination of Dynamic Macromolecular Complexes by Single Particle Cryo-EM
Selected Speakers: A/Prof. Grant Jensen, Howard Hughes Medical Institute/Caltech, Pasadena, USA
'High-Throughput' Bacterial Cryotomography
15:00 Afternoon Tea
15:30 Session 6: Cont'd
16:00 Free Afternoon
18:30 Charter bus from UQ Centre to Cocktain Party & Conference Dinner venue
19:00 Pre-Dinner Cocktail Party: Mango Tree Terrace ❘ Hillstone St Lucia
19:30-23:00
21:30 Conference Plenary Lecture: Prof. Joachim Frank, Howard Hughes Medical Institute Investigator & Professor, Department of Biochemistry and Molecular Biophysics, and Department of Biology, Columbia University, New York, USA
Electron Tomography Has Come of Age: from Molecules to Organelles to Cells to Tissues
"At a time when tomograms can be obtained with the push of a button, when cells start to be inventoried in their entirety, and when artifact-free cutting of high-pressure frozen cells or tissues with ion beams has become possible, 'old-timers' of the craft experience a sense of living in the future. In my talk I will trace back to how we got here, but also try to extrapolate and speculate what surprises the next meetings might bring."
23:00 Charter bus from Hillstone St Lucia back to conference hotels
DAY 5: Wednesday | 4th February, UQ Centre, The University of Queensland
08:00 Breakfast (Lobby)
09:00 Session 7: New advances in automation and instrumentation for ET
Chair: Prof. Abraham (Bram) Koster, Leiden University Medical Centre, The Netherlands
Development of Tools for Correlative Cryo-Electron Tomography
Invited Speakers: Prof. Ken Downing, Lawrence Berkeley National Laboratory, Berkeley, USA
Instrumentation Advances for Electron Tomography
Prof. Mark Ellisman, National Center for Microscopy and Imaging Research, University of California San Diego, USA
Multi-Scale Correlated Light and Electron Microscopic Imaging of the Nervous System
Dr Alex Rigort, Max-Planck Institute of Biochemistry, Martinsried, Germany
Micro-Machining Cells for Cryo-Electron Tomography
Selected Speakers: Dr Marc Storms, FEI Electron Optics BV, Eindhoven, The Netherlands
Advances in Serial Block Face DualBeam Electron Microscopy for the Exploration of Cortical Circuits
Dr Hans Tietz, TVIPS GmbH, Gauting, Germany
Acquisition of Electron Tomography Tilt Series Using a Very Large Area 64 MegaPixel Fiber Optic Coupled CMOS Detector
Dr Kazuhiro Aoyama, FEI Company, Japan
STEM Tomography for Thick Biological Specimens
Alan Robins, E.A. Fischione Instruments Inc., Export, USA
Advances in Specimen Holder Technology and Specimen Geometry for Room Temperature Electron Tomography
Dr Jacob Wilbrink, Gatan Inc., USA
3D Microscopy from the Millimeter Scale to the Nanometer Scale
11:00 Morning Tea
11:30 Session 7: Cont’d
12:30 Lunch (Kiosk & Verandah)
13:45 Closing Session: Discussion & Remarks
Chair: Brad Marsh, Institute for Molecular Bioscience, The University of Queensland, Australia
15:00 End of Congress