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Interpretation of results is final and crucial step of quantitative analysis. We summarized the information necessary to properly interpret the results of calculations in a table*:
Coefficient | Standard values | Values indicating colocalization | Values indicating absence of colocalization |
Pearson's correlation coefficient (Rr) | From -1.0 to 1.0 | From 0.5 to 1.0 | From -1.0 to 0.5 |
Manders' overlap coefficient (R) | From 0 to 1.0 | From 0.6 to 1.0 | From 0 to 0.6 |
Overlap coefficients k1 and k2 | Vary | Close values (0.5 and 0.6, 0.8 and 0.9) | Distant values (0.5 and 0.9, 0.2 and 0.7) |
Colocalization coefficients m1 and m2 | Vary | Above 0.5 | Below 0.5 |
Colocalization coefficients M1 and M2 | Vary | Above 0.5 | Below 0.5 |
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| *Complete quantitative colocalization analysis protocol can be found in Current Protocols in Cell Biology. | |
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